Replication Process of the Parvovirus H-1 II. Isolation and Characterization of H-1 Replicative Form DNA
نویسندگان
چکیده
منابع مشابه
DNA polymerase requirements for parvovirus H-1 DNA replication in vitro.
An in vitro system using nuclei from parvovirus H-1-infected cells was used to characterize the influence of inhibitors of mammalian DNA polymerases on viral DNA synthesis. The experiments tested the effects of aphidicolin, which is highly specific for DNA polymerase alpha, and 2',3'-dideoxythymidine-5'-triphosphate (ddTTP), which inhibits cellular DNA polymerases in the order gamma greater tha...
متن کاملPREPARATION AND CHARACTERIZATION OF [Ni (MAP) ] ; H- MAP = 2- (METHYLAMINO) PYRIDINE H. Aghabozorg* and H. H. Monfared
We have recently synthesized [Ni (MAP) ] complex [H-MAP= 2- (methylamino) pyridine]. 2- (Methy1amino)pyridine reacts with MeLi in THF and generates the lithium salt (Li-MAP). Further reaction of NiCl with two equivalent of this ligand in THF leads to the formation of a dark red solution. Analytical data, molecular weight, and magnetic susceptibility of this complex confirm the formula [N...
متن کاملSYNTHESIS OF 1,4,8 - TRIHYDROXY- 9 (1 0 H) - ANTHRACENONE
The title compound has been synthesized starting from 2-methoxybenzdiethylarnide (1).
متن کاملReplication of the parvovirus MVM. II. Isolation and characterization of intermediates in the replication of the viral deoxyribonucleic acid.
The time course of the appearance of intracellular viral DNA has been studied in mouse L cells infected with the single-stranded DNA virus MVM (minute virus of mice) by using a selective extraction procedure. Approximately half of this DNA elutes from hydroxyapatite as single-stranded DNA. It is sensitive to Escherichia coli exonuclease I and shows a sedimentation profile similar to DNA from th...
متن کاملChanges in the H-1 histone complement during myogenesis. II. Regulation by differential coupling of H-1 variant mRNA accumulation to DNA replication
We have shown that changes in proportions of the four chicken H-1's during in vitro myogenesis are primarily the result of differential coupling of their synthesis to DNA replication (see the previous paper). We show here that the four major chicken H-1's are encoded by distinct mRNAs which specify primary amino acid sequence variants. Accumulation of the H-1-variant mRNAs is coupled to DNA rep...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Virology
سال: 1974
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.13.2.400-410.1974